Flow-cytometric assessment of regulatory T cells
| Treatment | WEHI-164 | K7M2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD4+CD25+ (% ± SE) | FoxP3+CD25+ (% ± SE) | CD4+CD25+ cell no. (×106) ± SE | CD4+CD25+ (% ± SE) | FoxP3+CD25+ (% ± SE) | CD4+CD25+ cell no. (×106) ± SE | ||||||
| Naive | 4.8 ± 0.7 | 4.38 ± 0.06 | 0.21 ± 0.04 | 4.8 ± 0.7 | 4.38 ± 0.06 | 0.21 ± 0.04 | |||||
| Untreated | 6.0 ± 0.9 | 5.30 ± 0.2 | 0.37 ± 2.6 | 7.0 ± 0.2 | 5.90 ± 0.3 | 0.44 ± 0.3 | |||||
| G-G | 4.9 ± 0.6 | 3.70 ± 0.5 | 0.06 ± 0.9*** | 4.6 ± 0.3 | 2.70 ± 0.2 | 0.29 ± 0.2*** | |||||
| L-M | 4.1 ± 0.1 | 2.30 ± 0.9 | 0.09 ± 0.2*** | 3.7 ± 0.3 | 3.05 ± 0.5 | 0.28 ± 0.2*** | |||||
| G-L-M-G | 4.3 ± 0.3 | 3.00 ± 0.2 | 0.06 ± 0.9*** | 5.1 ± 0.3 | 4.50 ± 0.1 | 0.32 ± 0.2*** | |||||
| L-M-G-G | 2.4 ± 0.3 | 2.54 ± 0.8 | 0.08 ± 0.6*** | 4.1 ± 0.3 | 3.20 ± 0.5 | 0.21 ± 0.1*** | |||||
CD4+CD25+ Treg cells assessment in tumor-draining lymph nodes after therapeutic treatments.
The modulation of CD4+CD25+ and FoxP3+CD25+ double-positive Treg cells from tumor-draining lymph nodes was evaluated in naive-treated or untreated WEHI-164 and K7M2 tumor-bearing mice 3 days after the term of all the therapeutic treatments. The results are indicated in percentage (%) form and absolute number (cell no.) (mean ± SE). Data are representative for at least three mice belonging to the treatment group.
***
***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05.