Fig. 2.
Inactivation of Pax7 in adult satellite cells markedly impairs muscle regeneration. (A) Schematic showing the experimental regime for single injury used in this study. (B) H&E staining of tibialis anterior muscle 21 d after acute injury induced by CTX injection. Arrowheads point to fat depositions. (C) Immunostaining for perilipin (in green) demonstrates increased adipogenesis in Pax7fl/CreERT2 mice, laminin staining is shown in red, and nuclei are counterstained with DAPI (in blue). (D) Schematic showing the experimental regime for double CTX injury used in this study. (E) H&E staining of tibialis anterior muscle 21 d after the second injury induced by CTX injection. Arrowheads point to fat depositions. (E’) A magnification of E. (F) Immunostaining for perilipin (in green) demonstrates increased adipogenesis in Pax7fl/CreERT2 mice, laminin staining is shown in red, and nuclei are counterstained with DAPI (in blue). (Scale bar: 100 µm.)