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. 2013 Sep 24;110(41):16474–16479. doi: 10.1073/pnas.1307680110

Fig. 3.

Fig. 3.

Significantly impaired muscle regeneration with continuous tamoxifen treatment. (A) H&E staining 10 d after acute injury reveals markedly impaired regeneration following continuous tamoxifen treatment. (Scale bar: 100 μm.) (B) Immunostaining for laminin (in red) showing decreased numbers of fibers and also reduced fiber feret. Nuclei are counterstained with DAPI (in blue). (Scale bar: 100 μm.) (C) Quantification of the minimal fiber feret of muscles at 10 d after acute injury; n= 6, ***P < 0.001. (D) Quantification of the amount of regenerating fibers at 10 d after CTX injury; n = 6, *P < 0.01. (E) Immunostaining for smooth muscle actin (SMA, in red) demonstrating increased numbers of SMA-positive nuclei in Pax7fl/CreERT2 animals compared with Pax7fl/+ animals at 10 d after acute injury. Nuclei are counterstained with DAPI (in blue). (Scale bar: 100 μm.) (F) Quantification of SMA-positive nuclei relative to the total number of nuclei; n = 6, *P < 0.05. (G) Quantification of the numbers of interstitial cells, n = 4, ***P < 0.001.