Fig. 3.
Postnatal maturation of Bergmann glia and Purkinje neurons in Gpr37l1+/+ and Gpr37l1−/− male mice. (A) Immunofluorescence labeling of Glast in P10 and P15 cerebella of Gpr37l1+/+ and Gpr37l1−/− littermates. (B) Immunofluorescence labeling of Calb in P10, P15, and P20 cerebella of Gpr37l1+/+ and Gpr37l1−/− littermates. (C) Quantification of Calb immunostaining intensity. Data are expressed in arbitrary units (mean ± SEM, n = 3 per group), *P = 0.057, **P < 0.001 +/+ vs. −/−, unpaired t test. (D) Double immunofluorescence staining of VGluT1 and VGluT2 in P10, P15, and P20 cerebella of Gpr37l1+/+ and Gpr37l1−/− littermates. (Scale bars in A, B, and D, 25 μm.) EGL, external granular layer; IGL, internal granular layer; ML, molecular layer. Asterisks indicate Purkinje neuron somata.