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. 2013 Sep 23;110(41):16486–16491. doi: 10.1073/pnas.1314819110

Fig. 4.

Fig. 4.

Postnatal cerebellar Shh signaling and Gpr37l1–Ptch1 interaction in Gpr37l1+/+ and Gpr37l1−/− male mice. (A) Immunoblotting and densitometric quantification of Shh pathway proteins in whole cerebellar extracts prepared at P5, P10, and P15 from Gpr37l1+/+ and Gpr37l1−/− littermate pups (mean ± SEM, n = 3 mice per group), *P < 0.05, **P < 0.005 +/+ vs. −/−, unpaired t test. (B) Double immunofluorescence staining of Gpr37l1 and Ptch1 in sagittal cerebellar sections of P3 wild-type pups. (C and D) Coimmunoprecipitation analysis of Ptch1 and Gpr37l1 in cerebellar extracts from P10 Gpr37l1+/+ and Gpr37l1−/− littermates. Immunoprecipitations (IP) were performed with the Ptch1 or Gpr37l1 antibody followed by immunoblotting for the indicated proteins. A representative result from three distinct experiments is shown. (Scale bars, 10 μm.) Asterisks indicate Purkinje neuron somata; arrows point to doubly positive Bergmann glia cells.