Skip to main content
. 2013 Sep 25;110(41):16669–16674. doi: 10.1073/pnas.1316622110

Fig. 2.

Fig. 2.

UVR8–COP1–SPA complexes are UV-B– and COP1–dependent. (A and B) Co-IP assays using 4-d-old Col, TAP-SPA1 (A) and TAP-SPA3 (B) seedlings grown under –UV-B and +UV-B. (C) Photomorphogenic UV-B–induced hypocotyl growth of spa mutant seedlings. (Upper) Phenotypes of 4-d-old seedlings of indicated genotypes grown under –UV-B and +UV-B. (Lower) Quantification of relative hypocotyl length. (D) The expression of photomorphogenic UV-B–responsive genes in 4-d-old Col, cop1-4, and spa mutant seedlings. (E and F) Co-IP assays using 4-d-old Wassileskija (Ws), YFP-COP1/cop1-5 (E), Col, and FLAG-UVR8 (F) seedlings grown under –UV-B and +UV-B. (G) The interaction among SPA, COP1, and UVR8 in yeast. (Left) Yeast three-hybrid assays expressing indicated proteins under –UV-B and +UV-B. (Right) Quantification of β-galactosidase activity of yeast grown under –UV-B and +UV-B for 16 h.