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. 2013 Sep 10;110(41):16568–16573. doi: 10.1073/pnas.1308755110

Fig. 4.

Fig. 4.

Pharmacological HIF1α ablation antagonizes Ovx-induced bone loss and osteoclast activation. (A) Suppression of HIF1α protein expression in Raw264.7 cells by E2 or 2ME2 in hypoxic conditions. (B) Effects of E2 or 2ME2 on Hif1α mRNA levels in Raw264.7 cells. Data represent mean Hif1α expression relative to that of Actb ± SD (n = 5). (C, Top, Middle, and Bottom) BMD of femurs from Sham or Ovx treated with vehicle or 2ME2. (D) ES/BS of femurs from Sham or Ovx treated with vehicle or 2ME2. (E) Serum CTx of Sham or Ovx mice treated with vehicle or 2ME2. Error bars indicate means ± SD (n = 5). *P < 0.05; **P < 0.01; ***P < 0.005; NS, not significant.