Skip to main content
. 2013 Sep 19;110(41):16550–16555. doi: 10.1073/pnas.1310215110

Fig. 1.

Fig. 1.

Expression of RAE-1γ by an MCMV vector improves the specific CD8 T-cell response to a vectored antigen. (A) RAE-1γMCMVList and RAE-1γMCMV-SIINFEKL were constructed on the RAE-1γMCMV backbone by replacing the Dd-restricted antigenic m164 peptide 167AGPPRYSRI175 with either the Kd-restricted listeriolysin O (LLO)-derived peptide 91GYKDGNEYI99 or the H-2Kb–restricted ovalbumin-derived peptide SIINFEKL. (B) BALB/c mice were infected i.v. with 2 × 105 pfu per mouse of the indicated viruses, and viral titers were determined by plaque assay. DL, detection limit. (C) Splenocytes of MCMVList- or RAE-1γMCMVList–infected BALB/c mice (105 pfu per mouse, via f.p.) were stained for LLO tetramer-specific CD8 T cells. (D) C57BL/6 mice were f.p. infected with 105 pfu per mouse of MCMV-SIINFEKL or RAE-1γMCMV-SIINFEKL. At indicated time points, splenocytes were isolated, stimulated with SIINFEKL-peptide, and stained for the intracellular IFN-γ production. For panels B–D, individual animals (circles or triangles) and median values are shown.