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. 2013 Jul 30;41(19):9033–9048. doi: 10.1093/nar/gkt654

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — In vitro growth of mutants with transposon insertions in sick genes. (A) Culture density (OD 600) of wild-type V. cholerae and mutants with transposon insertions in ‘sick’ gene candidates is plotted as a function of time. (B) Competitive indices from in vitro competition assays between wild-type V. cholerae and mutants with insertions in candidate ‘sick’ genes are shown. Competitive indices were calculated as the ratios of mutant and wild-type CFUs after overnight co-culture in LB. *P < 0.001.