Figure 2.

Frequencies of spontaneous mutations in the CAN1 locus in strains with compromised ROS-scavenging activities. Mutation frequencies at the CAN1 locus were calculated as described in Materials and Methods section. Average mutation frequency measured for at least six independent cultures incubated at 37°C for 3.5 h to induce telomere un-capping and at 23°C in control experiments. Asterisk indicates statistically significant difference (P < 0.005) in frequencies of mutations as determined by Mann–Whitney test. NS indicates the absence of significant differences.