Figure 3.

Exogenous oxidative damage induces mutagenesis in ssDNA. Mutation frequencies at the CAN1 locus were calculated as described in Materials and Methods section. Average mutation frequency measured for at least four independent cultures incubated at 37°C for 3.5 h induce telomere uncapping followed by exposure to 5 mM hydrogen peroxide or mock treatment are presented. Mid-chromosome reporter (described in Results section) was used as a control under the same experimental conditions. Numbers above the bars reflect the absolute frequency of mutation, numbers with ‘+’ indicate how many additional mutations were deposited at CAN1 locus following exposure to hydrogen peroxide. Standard deviations of average are presented in Supplementary Table S4.