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Estimated frequencies of spontaneous and oxidative stress-induced GCRs. Frequencies of GCRs were calculated as the ratio of the number of CanR colonies unable to grow on the media lacking uracil to the total number of cells in the culture. Median frequency of Ura− CanR colonies per genotype per experimental condition for at least four independently selected cultures is presented. Asterisk indicates statistically significant differences (Mann–Whitney test, P < 0.03).
