Figure 6.

Mutagenic potential of the nucleotides and role of TLS Pol zeta in ROS-induced mutagenesis. Propensity of the pre-mutagenic lesions to cause spontaneous and oxidative stress-induced mutations and the role of TLS in the mutagenesis is schematically depicted. Fraction of the mutations occurred at each nucleotide calculated from Supplementary Table S1 for each experimental condition. The height of corresponding letter is proportional to the contribution of each nucleotide to ssDNA mutagenesis. Bar graph reflects the relative frequencies of mutations at CAN1 locus in ssDNA reporter for each experimental condition in wt (Rev3+) and rev3Δ (Rev3−) strains.