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Catalytic efficiency for extension beyond a mispair by wt RB69pol and L415 variants
| Parameters | wt | L415A | L415G | L415M |
|---|---|---|---|---|
| kpol (s−1) | N.A.D | 19 ± 1 | 46 ± 4 | N.A.D |
| Kd (µM) | >2000 | 245 ± 30 | 1300 ± 270 | >2000 |
| kpol/Kd (µM−1 s−1) | 8.1 × 10−4 | 7.7 × 10−2 | 3.5 × 10−2 | 4 × 10−3 |
N.A.D stands for not accurately determined because the corresponding Kd,app value is greater than 2 mM, and therefore only the kpol/Kd,app value is reliable.
