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FRET efficiency for tCo-tCnitro pair in binary complexes with wt RB69pol and L415 variants
| P/T | P/T alone | wt-P/T | L415A-P/T | L415G-P/T | L415M-P/T |
|---|---|---|---|---|---|
| Con-P/T | 0.43 ± 0.01 | 0.37 ± 0.01 | 0.20 ± 0.02 | 0.19 ± 0.02 | 0.35 ± 0.05 |
| Mis-P/T | 0.32 ± 0.04 | 0.18 ± 0.04 | 0.23 ± 0.02 | 0.21 ± 0.02 | 0.24 ± 0.05 |
| P-value | 0.01 | 0.002 | 0.07 | 0.18 | 0.04 |
Representative fluorescence spectra of various tCo-containing P/Ts in the absence and presence of pol are shown in Supplementary Figure S4.
