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. 2013 Aug 7;41(19):9006–9019. doi: 10.1093/nar/gkt688

Figure 1.

Figure 1.

Htz1 is required for cell survival after UV and global CPD repair in the overall genome. (A) The UV sensitivity of wild-type cells and of cells lacking the HTZ1, SWR1 or GCN5 genes. Data are the average of at least three independent experiments ± SD. (B) Repair of CPDs from the genome. DNA from samples taken before and after UV (100 J/m2) was treated with a CPD-specific endonuclease. The digestion products were then separated by gel electrophoresis under denaturing conditions and visualized with EtBr. Here, 0, 0.5, 1, 2 and 3 indicate repair times (hours). (C) CPD content in genomic DNA. The gel in (B) was scanned using a Typhoon Trio (GE Healthcare Life Sciences), and the median migrating distances of the DNA smear were calculated using ImageQuant 5.0. CPD content (CPDs/kb) was calculated as described by Sutherland and Shih (49) and Bespalov et al. (50). The data were fitted to a second-order polynomial. Values represent the mean ± SD of three independent experiments.