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. 2013 Aug 7;41(19):9006–9019. doi: 10.1093/nar/gkt688

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Htz1 acetylation at sites K3, 8, 10, 14 is not required for UV survival and CPD repair. (A) The sensitivity to UV irradiation of strains with wild-type HTZ1 or htz1 alleles in which the 4 N-terminal acetylation sites have been substituted by arginine or glutamine residues. Data are the average of at least three independent experiments ± SD. (B) CPD levels in the genome were detected by the slot-blot assay. DNA from samples taken after UV (100 J/m²) was probed with CPD-specific antibodies. Indicated on the left side of the image are the repair times in hours. CPD levels are presented relative to the amount in samples taken immediately after UV irradiation. Data were the average of at least three independent experiments ± SD, and fitted to a second-order polynomial.