Table 1.
Off-target sites for ZFNs targeted to CCR5 displaying significant evidence of ZFN induced indels grouped by classifier probability score
| Probability score | Genomic targets with ZFN indels over total targets screened |
Percentage of targets with significant evidence of ZFN-induced indelsa | Number of targets in human genome scored in this range | |
|---|---|---|---|---|
| Non-exons | Exons | |||
| 0–0.1 | 0 | 1/1 | 100% | 1 |
| 0.1–0.2 | 1/4 | 1/1 | 40% | 5 |
| 0.2–0.3 | 6/12 | 1/1 | 54% | 60 |
| 0.3–0.4 | 2/12 | 1/6 | 17% | 241 |
| 0.4–0.5 | 4/12 | 1/13 | 20% | 816 |
| 0.5–0.6 | 2/12 | 2/11 | 17% | 2155 |
| 0.6–0.7 | 0/13 | 0/13 | 0% | 5947 |
| 0.7–0.75 | 0/13 | 1/13 | 4% | 6657 |
aSignificant evidence of ZFN-induced indels as compared with background (controls receiving plasmid with no zinc fingers) was determined using a Fishers exact test and a P-value of 0.05.