Figure 6.

Application of the assay to K-562 nuclear extracts and other C5 DNMTs. (A) Increasing amounts of NCE and SNE from K-562 cells have been tested for DNA methylation activity using Dup_1 hmC and nmC (Table 1). The experiment was performed in duplicates. Means are represented with standard deviation. (B) Two independent dose-response curves for sinefungin on K-562 SNE, each in duplicates. (C) Two independent dose-response curves for sinefungin on DNMT3A-C, each in duplicates. (D) Two independent dose-response curves for sinefungin on M.SssI, each in duplicates. Means of IC₅₀ and coefficient of determination (R²) are displayed.