Table 1.

Sequences of tested duplexes.

Duplex Name	Number of CpG	Sense	Anti-sense
Dup_0	8	5′-GATmCGCmCGATGmCGmCGAATmCGmCGATmCGATGmCGAT-3′	5′-ATCGCATCGATCGCGATTCGCGCATCGGCGATC-3′
Zebularine-containing duplex	8	5′-GATmCGCmCGATGmCGmCGAATmCGmCGATmCGATGmCGAT-3′	5′-ATZebGCATZebGATZebGZebGATTZebGZebGCATZebGGZ ebGATC-3′
Dup_1 hmC	8	5′-GATmCGCmCGATGmCGmCGAATmCGmCGATmCGATGmCGAT-3′	BIOT-5′-ATCGCATCGATCGCGATTCGCGCATCGGCGATC-3′
Dup_1 nmC	8	5′-GATCGCCGATGCGCGAATCGCGATCGATGCGAT-3′	BIOT-5′-ATCGCATCGATCGCGATTCGCGCATCGGCGATC-3′
Dup_2 hmC	1	5′-GCTATATATACGTACTGTGAACCCTACCAGACATGCACTG-3′	BIOT-5′-CAGTGCATGTCTGGTAGGGTTCACAGTAmCGTATA TATAGC-3′
Dup_2 nmC	1	5′-GCTATATATACGTACTGTGAACCCTACCAGACATGCACTG-3′	BIOT-5′-CAGTGCATGTCTGGTAGGGTTCACAGTACGTAT ATATAGC-3′
Dup_3	1	5′-GCATATATATGAmCGATCCTGTAGGTCACTACCAGACATGCACTG-3′	BIOT-5′-CAGTGCATGTCTGGTAGTGACCTACAGGATCGTCA TATATATGC-3′
Dup_4	1	5′-GGAGGCCmCGCCTGCTGTAGGTCACTACCAGACATGCACTG-3′	BIOT-5′-CAGTGCATGTCTGGTAGTGACCTACAGCAGGCGG CCTCC-3′
Dup_5	1	5′-TCCTGTGAGCCTCCmCGCAGGTCACTACCAGACATGCACTG-3′	BIOT-5′-CAGTGCATGTCTGGTAGTGACCTGCGGAGGCTC ACAGGA-3′

hmC, hemi-methylated cytosine; nmC, non-methylated cytosine; BIOT, biotin; Zeb, zebularine.