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. 2013 Aug 8;5(9):1351–1366. doi: 10.1002/emmm.201202183

Figure 5. PRL-3 promotes growth and suppresses apoptosis of TF-1 leukaemia cells upon cytokine deprivation.

Figure 5

  1. Right panel: MTS assay results reflecting numbers of viable TF1-GFP and TF1-PRL-3 cells after culture in the absence of cytokines for various durations. Error bars represent the mean ± SD from three independent experiments. Left panel: Western blot analysis of TF1-GFP and TF1-PRL-3 cells. GAPDH, loading control.
  2. Flow cytometry analysis of propidium iodide-stained TF1-GFP and TF1-PRL-3 after 48 h culture in the absence of cytokines. Note the difference in sub-G1 peak/population, reflective of apoptotic cells. Representative data from three independent experiments are shown.
  3. Left panel: flow cytometry analysis of annexin-V- and 7-AAD-stained TF1-GFP and TF1-PRL-3 after 48 h culture in the absence of cytokines. The percentage in the upper left quadrant indicates the fraction of annexin-V-positive apoptotic cells in the entire cell population analysed. Right panel: quantitation of annexin-V-positive apoptotic population in three independent experiments. Statistical differences between two groups were determined using Student's t-test (mean ± SD, n = 3, ***p = 0.0012).