Figure 6. Overexpression of miR-382 influences responses of MSNs in NAc slices to DRD1 activation: 4 µl of vehicle, Ad-GFP or Ad-miR-382 (1 × 10⁹ pfu/ml) was infused into the NAc of rats.

Severn days later, the NAc were isolated.

• A-C. The successful modulation of miR-382 expression by Ad-miR-382. Ad-miR-382 increased the expression of miR-382 in NAc of rats (p = 0.00125). Values are mean ± SEM from 3 independent experiments (n = 3), compared with that in Ad-GFP control. *p < 0.01, Student's t-test. Sample voltage traces in response to current injections (inset) from a MSN in acute brain slices of rats which received infusion of saline (B) and Ad-miR-382 (C), respectively. While the application of 1 µM SKF38393, the DRD1 agonist increased the firing rate of the MSN of rats that received saline injection, this was not seen in rats received Ad-miR-382 injection.
• D. Summary graphs showing that Ad-miR-382 NAc injection attenuated the firing rate of MSNs–induced by 1 µM SKF38393 (p = 0.00654). Values are mean ± SEM from 4 independent experiments (n = 4), compared with that in Ad-GFP control. **p < 0.01, two-way ANOVA.