Table 1.
The aberrantly expressed miRNAs in NAc from rats after treatment with alcohol. Source data is available for this figure in the Supporting Information
| miRNAs | Vehicle | Alcohol | Alcohol/vehicle | miRNAs | Vehicle | Alcohol | Alcohol/vehicle | ||
|---|---|---|---|---|---|---|---|---|---|
| Downregulated | Mean signal | Mean signal | % Change | p-value | Upregulated | Mean signal | Mean signal | % Change | p-value |
| rno-miR-379 | 3208 | 2309 | 72 | 2.24E-07 | rno-miR-29b | 274 | 4365 | 1595 | 6.37E-06 |
| rno-miR-409-3p | 684 | 487 | 71 | 8.23E-05 | rno-miR-204 | 86 | 844 | 977 | 4.81E-05 |
| rno-miR-181b | 2734 | 1882 | 69 | 8.77E-04 | rno-miR-27a | 460 | 1682 | 366 | 2.17E-05 |
| rno-miR-539 | 1991 | 1297 | 65 | 6.98E-10 | rno-miR-335 | 562 | 2016 | 359 | 1.23E-04 |
| rno-miR-25 | 896 | 583 | 65 | 3.51E-05 | rno-miR-30a | 1425 | 4401 | 309 | 8.15E-06 |
| rno-miR-376b-5p | 435 | 272 | 62 | 1.35E-04 | rno-miR-29c | 3350 | 9424 | 281 | 2.56E-05 |
| rno-miR-15b | 419 | 254 | 61 | 3.03E-05 | rno-miR-101a | 174 | 467 | 268 | 2.14E-05 |
| rno-miR-382 | 4463 | 2621 | 59 | 7.13E-11 | rno-miR-22 | 562 | 1467 | 261 | 1.04E-08 |
| rno-miR-433 | 4454 | 2614 | 59 | 6.61E-11 | rno-miR-30e | 665 | 1662 | 250 | 8.09E-08 |
| rno-miR-320 | 3629 | 2126 | 59 | 2.91E-12 | rno-miR-100 | 1147 | 2089 | 182 | 1.43E-04 |
| rno-miR-93 | 530 | 307 | 58 | 1.80E-08 | rno-miR-99a | 1374 | 2362 | 172 | 2.93E-04 |
| rno-miR-151 | 5210 | 2994 | 57 | 1.16E-11 | rno-miR-153 | 1316 | 2164 | 164 | 3.76E-06 |
| rno-miR-361 | 7181 | 4126 | 57 | 1.27E-12 | rno-miR-376b-3p | 672 | 1072 | 160 | 3.79E-03 |
| rno-miR-92b | 4387 | 2436 | 56 | 5.07E-03 | rno-miR-92a | 644 | 985 | 153 | 5.42E-04 |
| rno-miR-674-5p | 1213 | 656 | 54 | 9.59E-11 | rno-miR-145 | 1116 | 1666 | 149 | 1.90E-04 |
| rno-miR-674-3p | 455 | 245 | 54 | 3.74E-04 | rno-miR-137 | 6651 | 9225 | 139 | 9.67E-06 |
| rno-miR-129 | 671 | 357 | 53 | 5.66E-08 | rno-miR-30b-5p | 6317 | 8670 | 137 | 2.01E-04 |
| rno-miR-338* | 659 | 324 | 49 | 1.52E-08 | rno-miR-212 | 1065 | 1434 | 135 | 3.65E-03 |
| rno-miR-760-3p | 501 | 238 | 47 | 8.29E-10 | rno-miR-29a | 16,672 | 22,118 | 133 | 1.41E-07 |
| rno-miR-324-5p | 836 | 376 | 45 | 3.20E-08 | rno-miR-352 | 2631 | 3430 | 130 | 3.29E-03 |
| rno-miR-330* | 834 | 366 | 44 | 1.55E-13 | |||||
| rno-miR-346 | 1086 | 448 | 41 | 1.81E-12 | |||||
| rno-miR-485 | 1326 | 546 | 41 | 1.45E-13 | |||||
| rno-let-7d* | 1337 | 513 | 38 | 8.63E-08 | |||||
| rno-miR-134 | 1214 | 362 | 30 | 9.10E-14 | |||||
| rno-miR-122 | 687 | 87 | 13 | 5.66E-08 | |||||
Eighteen male rats (weighing 150–180 g) were divided into two treatment groups (n = 9): vehicle (500 µl saline, i.p. bid) or alcohol (1 g/kg, i.p. bid). Seven days later, the animals were sacrificed and their NAc were isolated for miRNA microarray analysis. miRNAs that were highly expressed in NAc and over 30% changes (p < 0.05, Student's t-test) in their expression after alcohol treatment were listed.