Table 2.
Serological analysis of sera that were positive in the Flavi-blocking ELISA †.
| Sample ID † | Age * | Blocking ELISA # | Virus Neutralization Assay Titres | |||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Flavi (4G2) | MVEV (10C6) | KUNV (3.1112G) | MVEV | KUNV | ALFV | STRV | KOKV | Neutralization Diagnosis₸ | ||
| R1 | 5 | + | − | − | − | − | − | − | 20 | KOKV |
| R10 | 10 | + | + | + | − | − | ND | ND | ND | unknown |
| R15 | 5 | + | − | − | − | − | − | − | − | unknown |
| R21 | 12 | + | − | − | − | − | − | − | 80 | KOKV |
| R26 | 12 | + | − | − | − | − | − | − | 40 | KOKV |
| R41 | 4 | + | − | − | − | − | − | − | 40 | KOKV |
| R44 | 10 | + | − | − | − | − | − | − | 80 | KOKV |
| R5 | 9 | + | − | − | − | − | − | − | 20 | KOKV |
| R7 | 5 | + | − | − | − | − | 20 | − | − | ALFV |
| T101 | 14 | + | − | + | − | 80 | − | − | 40 | KUNV/KOKV |
| T103 Ɣ | 11 | + | − | − | − | − | − | − | 40 | KOKV |
| T104 | 18 | + | − | − | − | − | − | 80 | 40 | STRATV/KOKV |
| T105 | 15 | + | − | + | − | − | − | 80 | − | STRATV |
| T106 Ɣ | ≥8 | + | − | − | 20 | − | 160 | 80 | 40 | ALFV/STRATV |
| T107 | 15 | + | − | − | − | − | − | − | 20 | KOKV |
| T108 Ɣ | 35 | + | − | − | − | − | − | − | 40 | KOKV |
| T109 | 22 | + | − | − | − | − | − | − | − | unknown |
| T111 | 19 | + | − | − | − | − | − | 80 | 80 | STRATV/KOKV |
| T112 | 15 | + | − | − | − | 20 | − | − | 40 | KUNV/KOKV |
| T113Ɣ | 10 | + | − | − | − | − | − | 80 | 20 | STRATV |
| T114 Ɣ | 15 | + | + | − | − | − | − | 80 | − | STRATV |
| T115 Ɣ | 18 | + | + | + | 80 | − | 40 | 160 | 20 | STRATV/MVEV |
| T116 Ɣ | 15 | + | + | − | 40 | 40 | 40 | 160 | 40 | STRATV |
| T118 Ɣ | 19 | + | + | + | − | − | − | 80 | − | STRATV |
| T119 Ɣ | 15 | + | − | − | − | − | − | 160 | 20 | STRATV |
| T122 | 9 | + | − | − | − | − | − | − | − | unknown |
| T124 | 26 | + | − | − | − | − | − | 160 | − | STRATV |
| T125 | 19 | + | − | − | − | − | 40 | 40 | 20 | STRATV/ALFV/KOKV |
| T127 Ɣ | 14 | + | + | − | − | − | − | − | 20 | KOKV |
| T129 | 21 | + | − | + | − | − | − | − | − | unknown |
| T131 | ≥8 | + | − | + | − | − | − | − | − | unknown |
| T135 | 12 | + | − | − | − | − | 80 | − | 80 | ALFV/KOKV |
| T141 | 1 | + | + | − | 20 | − | 20 | − | 40 | MVEV/ALFV/KOKV |
| T142 | 21 | + | − | − | − | − | − | − | 20 | KOKV |
| T143 | 1 | + | + | − | 160 | − | − | − | 20 | MVEV |
| T145 | 7 | + | − | − | − | − | − | − | − | unknown |
| T146 | 4 | + | − | − | − | − | − | − | 40 | KOKV |
* Approximate age at the time of serum collection in 2011; # Blocking ELISA inhibition values ≥30 are considered positive, “+” positive; “−” negative; ₸ Virus neutralization titres that differ by ≥ 4 fold are considered significant; Ɣ Horses were part of a JEV vaccine trial in 2008 [21]; † The sera from 4 horses that were positive by the flavi blocking ELISA, but were not assessed in any of the other assays have not been included in this table; ND—not done; MVEV—Murray Valley encephaltitis virus; KUNV—West Nile virus (Kunjin virus); ALFV—Alfuy virus; STRATV—Stratford virus; KOKV—Kokobera virus.