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Representative cAMP BRET assay for Neuro-2A cells endogenously expressing mCB1 with 10 μM forskolin (F), forskolin plus 1 μM CP55,940 (F + CP) and, forskolin plus 1 μM SR141716A (F + SR), and (A) 1 μM ORG27569 (ORG) or (B) 0.1 μM PSNCBAM-1 (PSN) in the presence and absence of 1 μM CP55,940. Emission data for RLuc and YFP were collected over time and values plotted as raw ratio (± SEM) of emissions 460/535 over time (min). Data are a representative of three individual experiments.
