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. 2013 Oct 18;8(10):e77837. doi: 10.1371/journal.pone.0077837

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© 2013 Irague et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sequences are clustered according to the specificity of the glucansucrases. Catalytic (D400 and E438, DSR-S vardel Δ4N numbering) and transition state stabilizer (D511, DSR-S vardel Δ4N numbering) amino acids are highlighted in red; amino acids chosen for mutagenesis are highlighted in green.