Figure 4. The effect of PTP1B deletion on autophagic flux and NCK1 expression in skeletal muscle of obese mice.

Representative Western blots (a) and densitometric analysis (n = 6) of ATG5 (b), ATG7 (c), Beclin-1 (d), p62 (e), and NCK1 (f) protein levels in gastrocnemius muscles of the C57 or PTP1BKO mice received normal (ND) or high-fat content (HFD) diets for 20 weeks. ^* p<0.05 compared with C57 ND mice, ^† p<0.05 compared with C57 HFD mice, ‡ p<0.05 compared with PTP1BKO ND mice.