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. 2013 Oct 18;8(10):e77228. doi: 10.1371/journal.pone.0077228

Figure 7. The role of ROS in tunicamycin (TM)-induced PTP1B protein expression in C2C12 myotubes.

Figure 7

(a–d) Representative Western blots (a) and densitometric analysis (n = 6) of PTP1B (b), GRP78 (c), and phospho- and total-eIF2α (p-eIF2α and t-eIF2α, respectively) (d) protein levels in C2C12 myotubes treated with tunicamycin in presence of 10 mmol/l NAC for 24 hours. (e–g) Representative Western blots (e) and densitometric analysis (n = 6) of GRP78 (f), and p-eIF2α and t-eIF2α (g) protein levels in C2C12 myotubes treated with tunicamycin in presence of 1 mmol/l TUDCA for 24 hours. * p<0.05 compared with non-treated control cells, † p<0.05 compared with tunicamycin-treated cells.