Table 2. Comparison of MIC values and gene expression between Salmonella field isolates and their triclosan-selected mutants.
| Strains | Mutation in fabI |
MIC (µg/ml)a
|
x-fold change in gene expression (based on group means)
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TRC | GEN | KAN | CHX | CIP | TET | FLO | CHL | acrA | tolC | acrF | marA | ramA | soxS | fabI | |||
| S. Enteritidis 92 | 0.25 | 1 | 4 | 2 | 0.032 | 1 | 4 | 4 | |||||||||
| S. Enteritidis 92 mutant 2 | Gly93 – Val | 32 | 0.5 | 2 | 1 | 0.032 | 1 | 4 | 4 | 1.2 ± 0.04 | 0.8 ± 0.1 | 1.1 ± 0.03 | 2.1 ± 0.5 | 1.8 ± 0.4 | 0.5 ± 0.2 | 1.2 ± 0.3 | |
| S. Hadar 5 | 0.125 | 2 | 8 | 2 | 0.25 | 32 | 4 | 4 | |||||||||
| S. Hadar 5 mutant 1 | Gly93 – Val | 16 | 1 | 4 | 2 | 0.25 | 32 | 4 | 4 | 1.1 ± 0.04 | 1.1 ± 0.2 | 0.9 ± 0.3 | 1.7 ± 0.2 | 2.8 ± 0.9 | 0.4 ± 0.1 | 1.4 ± 0.4 | |
| S. Infantis 1 | 0.125 | 1 | 4 | 2 | 0.25 | 128 | 4 | 4 | |||||||||
| S. Infantis 1 mutant 4 | Gly93 – Val | 64 | 0.5 | 2 | 1 | 0.25 | 128 | 4 | 4 | 1.2 ± 0.2 | 1.1 ± 0.1 | 1.2 ± 0.8 | 1.1 ± 0.2 | 0.9 ± 0.04 | 0.5 ± 0.1 | 1.9 ± 0.3 | |
| S. Livingstone 3 | 0.25 | 2 | 8 | 1 | 0.032 | 128 | 4 | 4 | |||||||||
| S. Livingstone 3 mutant 2 | Gly93 – Val | >64 | 1 | 4 | 0.5 | 0.032 | 128 | 4 | 4 | 1.5 ± 0.7 | 0.9 ± 0.02 | 0.9 ± 0.2 | 1.4 ± 0.2 | 1.2 ± 0.1 | 0.4 ± 0.1 | 1.3 ± 0.1 | |
| S. Paratyphi B | 0.125 | 1 | 4 | 4 | 0.125 | 64 | 4 | 4 | |||||||||
| S. Paratyphi B mutant 1 | Gly93 – Val | 32 | 0.5 | 2 | 2 | 0.125 | 64 | 4 | 4 | 0.9 ± 0.1 | 0.9 ± 0.2 | 0.9 ± 0.1 | 2.5 ± 1.1 | 1.2 ± 0.4 | 0.6 ± 0.2 | 12.4 ± 2.1 | |
| S. Saintpaul 80969 | 0.5 | 32 | 128 | 8 | 1 | 2 | 8 | 8 | |||||||||
| S. Saintpaul 80969 mutant 8 | Gly93 – Val | >64 | 16 | 64 | 4 | 1 | 2 | 8 | 8 | 1.5 ± 0.7 | 1.01 ± 0.1 | 0.9 ± 0.3 | 2.4 ± 0.7 | 1.1 ± 0.1 | 0.3 ± 0.1 | 1.5 ± 0.2 | |
| S. Typhimurium var. Cop. | 0.25 | 2 | 8 | 2 | 0.125 | 1 | 2 | 2 | |||||||||
| S. Typhimurium var. Cop. mutant 1 | Gly93 – Val | 64 | 0.5 | 4 | 2 | 0.125 | 1 | 2 | 2 | 0.9 ± 0.3 | 1.1 ± 0.1 | 0.8 ± 0.1 | 1.2 ± 0.3 | 1.1 ± 0.4 | 1.1 ± 0.2 | 1.2 ± 0.1 | |
| S. Virchow 2 | 0.25 | 4 | 8 | 4 | 0.25 | 64 | 4 | 256 | |||||||||
| S. Virchow 2 mutant 1 | Gly93 – Val | 16 | 1 | 2 | 4 | 0.25 | 64 | 4 | 256 | 1.9 ± 0.4 | 1.1 ± 0.1 | 1.2 ± 0.2 | 2.1 ± 1.1 | 3.4 ± 0.4 | 0.4 ± 0.2 | 1.5 ± 0.2 | |
a Antimicrobial agents and biocides are abbreviated as follows: CHL = chloramphenicol; CIP = ciprofloxacin; CHX = chlorhexidine; GEN = gentamicin; KAN = kanamycin; TET = tetracycline; TRC = triclosan