Fig. 6.

Left: Optical image of a Drosophila head. Center and Right: 3D reconstruction of a Drosophila head received by tiling 20 separate stacks. We observed strong THG signal (green) as well as SHG signal (red) from different tissue structures. The SHG signal seemed to come from muscle tissues while the THG signal was generated from the other type of tissues including the outer layers which are rich in chitin. Center: The back view and Right: the front view of the head. The thickness of the head is 220µm and the size of the head in the xy-plane is about 1mm × 1mm. Media 3 ^{(2.8MB, AVI)} shows the 3D rendering of the Drosophila head. The frame rate was ~3s/frame.