Figure 4. PFE suppressed DP2-induced phosphorylation of MAPKs in BEAS-2B cells.

After 16 h-starvation, cells were pretreated with 5, 15, 30 and 50 µg/mL PFE for 1 h and then stimulated with 20 µg/mL DP2 for 30 min. The treated cells were lysed for analysis of phosphorylation by immunoblot using specific antibodies and chemiluminescence development. Quantitative data was performed by densitometric analysis and indicated as [phosphorylated protein/ total protein]. The ratios of GST treatments were used as internal control.