Skip to main content
. 2013 Oct 18;8(10):e77458. doi: 10.1371/journal.pone.0077458

Table 1. Primers used for RT-PCR and qPCR analysis.

Target gene RT-PCR qPCR
IL-4 Forward 5′-cagctcgaacacttt gaa-3′ 5′-ccaactgcttccccctctg-3′
Reverse 5′-tctcacctcccaactgct-3′ 5′-tctgttacggtcaactcggtg-3′
IL-5 Forward 5′-ctgaggattcctgttcctgt-3′ 5′-ctgcctacgtgtatgccatcc-3
Reverse 5′-caactttctattatccactc-3′ 5′-cattggctatcagcagagttcg-3′
IL-13 Forward 5′-gctcctcaatcctctcct gtt-3′ 5′-gaaggctccgctctgcaat-3′
Reverse 5′-gcaacttcaatagtcagg tcc-3′ 5′-tctgggtcttctcgatggca-3′
GM-CSF Forward 5′-gcatgtgaatgccatccagg-3′ 5′-cactgctgctgagatgaatgaaa-3′
Reverse 5′-gcttgtagtggctggccatc-3′ 5′-gtctgtaggcaggtcggctc-3′
IL-6 Forward 5′-atgaactccttctccacaagcgc-3′ 5′-gtagtgaggaacaagccagagc-3′
Reverse 5′-caagagccctcaggctggactg-3′ 5′-ggcatttgtggttgggtca-3′
IL-8 Forward 5′-agatattgcacgggagaa-3′ 5′-ctcttggcagccttcctgattt-3′
Reverse 5′-gaaataaaggagaaacca-3′ 5′-cgcagtgtggtccactctcaat-3′
MCP-1 Forward 5′-gctcgctcagccagatgcaat-3′ 5′-actctcgcctccagcatgaa-3′
Reverse 5′-tgggttgtggagtgagtgttc-3′ 5′-ttgattgcatctggctgagc-3′
GAPDH Forward 5′-accacagtccatgccatcac-3′ 5′-atgcctcctgcaccacca-3′
Reverse 5′-tccaccaccctgttgcttga-3′ 5′-ccatcacgccacagtttcc-3′