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. 2013 Oct 18;8(10):e78459. doi: 10.1371/journal.pone.0078459

Figure 4. Engagement of SIRL-1 prevents NETosis in response to SLE plasma.

Figure 4

A) NET release from healthy neutrophils exposed to SLE plasma visualized as extracellular DNA (Sytox Green) by live cell imaging (Video S1) (neutrophils are blue; NETs are green). Individual frames are shown. Scale bar, 10 µm. B) Representative images of netting healthy neutrophils in response to SLE plasma with or without ligation of SIRL-1. Scale bars, 50 µm. C) Results from multiple independent donors are given. Neutrophils from 4 healthy donors were cultured for 3 h with 20% plasma from either nonautologous healthy controls (n=4) or individuals with SLE (n=5 or 6) in the presence or absence of anti-SIRL-1 mAb. NET formation was analyzed by fluorescence microscopy and the amount of NET-DNA quantified. Average density of each pooled group±SEM is shown. ***p<0.0001, paired Student’s t test.