Figure 1. Total RNAP II levels decrease during WT HSV-1 KOS infection.

HeLa cells were either mock infected or infected with WT HSV-1 KOS or with an ICP27 null mutant, 27-GFP in which the ICP27 coding sequence was replaced with GFP. Cells lysates were prepared at 1, 3, 5, 7 and 9 h after infection and separated on a 5-15% linear gradient SDS-polyacrylamide gel. Western blots were probed with polyclonal antibody N20, which detects all forms of RNAP II The cellular protein YY1 served as the loading control.