Skip to main content
. 2013 Oct 18;8(10):e79007. doi: 10.1371/journal.pone.0079007

Figure 4. DRB and FVP altered the staining pattern of H5 antibody.

Figure 4

A) RSF cells were either mock infected or infected with WT HSV-1 KOS at an MOI of 10. Cells were either left untreated (no drug) or were treated with 100 µM DRB or 450 nM FVP as indicated. Cells were fixed at 8 h and stained with RNAP II antibody ARNA3, which recognizes all forms of RNAP II or phosphoserine-2 antibody H5. For the H5 no drug panels, cells from mock and KOS samples have been shown at higher magnification to show the nuclear staining patterns and are identified by white arrows. B-C) To account for cell toxicity, HeLa cells were either mock infected, or infected with HSV-1 KOS at an MOI of 1 for up to 16 hours as indicated. Infected cells were either untreated or treated with 100 µM DRB or 450 nM FVP. Cells were collected at 4 h intervals, stained with Trypan Blue vital dye, and counted in triplicate using a hemocytometer to estimate B) percent cell death and C) total cell counts.