Table 2. Quantitative comparison of metabolites found in aqueous extracts of brain tissues of F, HF and HS group mice.
| Metabolites | Integral in F group a (mean±std)*10-4 | Integral in HF group a (mean±std)*10-4 | Integral in HS group a (mean±std)*10-4 | % Average change (HF vs. HS)b | |r|(p d)(HF vs. HS) c (|r|>=0.53) | % Average change (HS vs. F) b | |r|(p d)(HS vs. F) c (|r|>=0.51) |
|---|---|---|---|---|---|---|---|
| ATP | 2.94±0.56 | 3.07±1.36 | 2.36±0.33 | 30.1 | 0.53(0.09) | -19.7 | 0.63(0.01) |
| Tyr | 3.30±0.31 | 4.28±0.59 | 3.97±0.59 | 7.8 | 0.18(0.17) | 20.3 | 0.67(0.01) |
| FMA | 3.10±0.74 | 2.94±1.21 | 1.88±0.30 | 56.3 | 0.63(0.01) | -39.4 | 0.67(0.00) |
| Gly | 258±26.0 | 279±21.5 | 236±19.1 | 18.2 | 0.62(0.04) | -8.5 | 0.84(0.00) |
| m-Ino | 99.1±8.08 | 94.5±7.41 | 88.7±7.9 | 6.5 | 0.64(0.08) | -10.5 | 0.78(0.01) |
| GPC | 201±12.3 | 163±14.3 | 146±11.6 | 11.6 | 0.59(0.01) | -27.4 | 0.93(0.00) |
| Pcho | 120±11.0 | 103±9.00 | 108±8.70 | -4.6 | 0.44(0.15) | -10.0 | 0.43(0.01) |
| Cho | 27.8±4.83 | 31.7±22.4 | 22.6±1.80 | 40.2 | 0.44(0.14) | -18.7 | 0.56(0.01) |
| Cr | 678±28.5 | 668±55.2 | 715±24.3 | -6.6 | 0.43(0.14) | 5.5 | 0.64(0.01) |
| GABA | 145±9.17 | 152±11.1 | 138±10.7 | 10.1 | 0.64(0.01) | -4.8 | 0.37(0.06) |
| Gln | 235±7.77 | 260±13.3 | 254±19.7 | 2.4 | 0.04(0.43) | 8.1 | 0.76(0.00) |
| Suc | 52.2±3.71 | 48.3±6.28 | 53.6±5.36 | -9.9 | 0.61(0.05) | 2.7 | 0.27(0.27) |
| Mal | 90.0±7.28 | 95.0±5.81 | 99.5±3.70 | -4.5 | 0.61(0.04) | 10.6 | 0.84(0.00) |
| NAD | 4.75±0.98 | 4.31±1.58 | 5.43±0.57 | -20.6 | 0.63(0.04) | 14.3 | 0.40(0.06) |
| NAA | 388±8.28 | 406±31.0 | 418±13.2 | -2.9 | 0.42(0.15) | 7.7 | 0.81(0.00) |
| Lac | 594±45.2 | 594±48.3 | 672±65.0 | -11.6 | 0.72(0.01) | 13.1 | 0.73(0.01) |
a The relative integrals of metabolites were determined from 1D 1H NMR analysis of brain aqueous extracts of each group mice.
b Values are represented as the fold-induction of peak integral between groups.
c The absolute values of correlation number extracted from the correlation plots of OPLS-DA models. The cutoff values are 0.53 in the correlation-loading plot of HF vs. HS (Figure S4A in File S1) and 0.51 in the correlation-loading plot of HS vs. F (Figure S4B in File S1).
d The p values were obtained from student's t-test.