Table 1. Polymorphisms in cDNA sequences from pro amplicons in 13 cancer cell lines and reference to 22 full-length or near full-length proviruses.
Nucleotide a | |||||||||||||||
Viral genome positionb | 3018 | 3171 | 3189 | 3219 | 3262 | 3297 | 3316 | 3412 | 3519 | 3641 | 3642 | 3657 | |||
Plurality c | A | T | A | G | A | G | A | C | G | C | T | C | |||
Cell lines | LNCaP | .G | .C | C | . | . | A | .G | . | . | T | .G | . | ||
DU145 | . | . | . | . | . | . | . | . | . | . | . | . | |||
PC3 | . | . | . | . | . | . | . | . | . | . | . | . | |||
C33A | n | n | n | n | n | n | n | n | n | n | n | n | |||
SiHa | . | . | . | . | . | . | . | . | . | . | . | . | |||
CaSki | . | . | . | . | . | . | . | . | . | . | . | . | |||
HeLa | . | . | . | . | . | . | . | . | . | . | . | . | |||
UPCISCC-90 | . | . | .C | . | . | . | . | . | . | . | . | . | |||
UMSCC-47 | . | . | . | . | . | . | . | . | . | . | . | . | |||
FaDu | . | . | . | . | . | . | . | . | . | . | . | . | |||
MDA-MB-468 | . | . | .C | . | . | . | . | . | . | . | . | . | |||
MDA-MB-231 | . | . | . | . | . | . | . | . | . | . | . | . | |||
MCF7 | .G | .C | C | . | . | .A | .G | . | . | .T | .G | . | |||
Virus | Type | Position | |||||||||||||
Homo sapiens specific | K101 | I | chr22∶18,926,187–18,935,361 | G | C | C | A | . | . | . | . | . | . | G | . |
K102 | I | chr1∶155,596,457–155,605,636 | . | . | . | . | . | . | . | . | . | . | . | . | |
K103 | I | chr10∶27,182,399–27,183,366 | G | C | C | . | . | . | . | . | . | . | . | . | |
K104 | II | chr5∶30,486,760–30,496,205 | G | C | . | . | . | A | G | T | . | . | . | . | |
K106 | I | chr3∶112,743,124–112,752,282 | G | C | C | . | . | A | G | . | . | T | G | . | |
K107 | I | chr5∶156,084,717–156,093,896 | G | . | . | . | . | . | . | . | . | . | . | G | |
K108 | II | chr7∶4,622,057–4,640,031 | G | C | C | . | . | A | . | . | . | . | G | . | |
K109 | II | chr6∶78,426,662–78,436,083 | G | . | C | . | . | . | G | . | . | T | G | . | |
K113 | II | chr19∶21,841,536–21,841,541 | G | . | C | . | . | A | . | . | . | . | G | . | |
K114 | II | chr11∶101,565,794–101,575,259 | G | C | C | . | . | A | . | . | . | . | . | . | |
K115 | II | chr8∶7,355,397–7,364,859 | – | . | . | . | . | A | . | . | . | T | G | . | |
K116 | I | chr3∶85,280,336–185,289,515 | G | C | C | . | G | . | . | T | A | . | G | . | |
K117 | II | chr12∶58,721,242–58,730,698 | G | C | C | . | . | A | . | . | . | . | . | . | |
K(I) | II | chr3∶125,609,302–125,618,439 | G | C | . | A | . | A | . | T | A | . | . | . | |
K4 | I | chr1∶75,842,771–75,849,143 | G | C | C | . | . | A | . | . | – | – | – | – | |
K60 | I | chr21∶19,935,621–19,940,996 | G | C | . | A | G | . | . | . | A | . | . | . | |
Shared with Pan and Gorilla | K105 | I | chrUn:gl000219∶175210–176178 | G | C | . | . | G | A | . | T | A | . | . | T |
K110 | I | chr1∶160,660,575–160,669,806 | G | C | G | . | G | A | . | T | . | . | . | . | |
K111 | I | chr11∶118,591,724–118,600,883 | G | C | . | . | G | A | . | T | . | . | . | . | |
K112 | II | chr10∶6,866,141–6,875,603 | G | C | G | . | T | A | . | T | . | . | . | T | |
K118 | I | chr3∶101,410,737–101,419,859 | G | . | G | A | G | . | G | T | A | . | C | T | |
K5 | I | chr4∶165,916,840–165,924,068 | G | C | . | A | G | A | . | T | A | . | . | T | |
K51 | I | chr19∶22,757,824–22,764,561 | G | C | G | . | G | A | . | T | A | . | . | T | |
K50F | II | chr19∶37,597,549–37,607,066 | G | C | . | A | G | A | G | T | . | . | . | . |
Notations: “.” = same as in consensus, “n” = non detectable, “.G” = primary and secondary peaks of approximately equal detection, “–” = absent in alignment.
Position in the reference HERV-K genome (K108).
Plurality sequence indicates the most common nucleotide at that position among amplicons from the 13 cell lines.