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. 2013 Oct 18;8(10):e78832. doi: 10.1371/journal.pone.0078832

Figure 6. The effect of GW843682X (GW) on the expression of TLR2, TLR4, and MyD88.

Figure 6

(A) The effect of GW on the gene expression of TLR2, TLR4, and MyD88. THP-1 cells were treated with 10 µM GW for the indicated time periods. The gene expression of TLR2, TLR4, and MyD88 was detected by qRT-PCR. * P < 0.05 compared with the non-treated group. (B) The effect of GW on the protein expression of TLR2 and TLR4. THP-1 cells were treated with 10 µM GW for 24 h. TLR2 and TLR4 protein expression was detected by FACS. (C) The effect of GW on the protein expression of MyD88. THP-1 cells were treated with 10 µM GW for the indicated time periods. The protein expression of MyD88 was detected by western blot. β-actin protein expression was detected as loading control.