Figure 1.

Depicts the overall framework and assembly of the gradient fabrication system. Schematic of the controlled variation in syringe pump rates coupled with a constant rate of cell infusion which ensures uniform cell density throughout the gradient A); a schematic for the three component (expandable to five) gradient fabrication system B); and an example of the gradient profile that can be generated depicted with a colorimetric gradient for visualization purposes C). The storage modulus can be controlled systematically enabling facile correlations of mechanical properties via position D). The positional values correlate well with values obtained from discrete hydrogel standards of known composition and mass fraction E). Mass fraction, pore size, transitional dimensions are controlled by defined solution mixtures pre-loaded in syringe pumps.