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. 2013 Oct 18;8(10):e78125. doi: 10.1371/journal.pone.0078125

Figure 3. Detection of receptor binding specificity of avian and human viruses by real-time PCR.

Figure 3

A microplate was immobilized with 200 ng/well of α2,3PGA or α2,6PGA and then blocked with 1% lipid-free BSA in PBS at 4 °C overnight. Avian IAV D313 (A) and human IAV M71 (B) (2° HAU, 50 μl/well) were incubated for 2 h at 4 °C on microplates that were immobilized with α2,3PGA or α2,6PGA. Viral RNAs of viruses bound to α2,3PGA or α2,6PGA were extracted. Viruses that bound to α2,3PGA or α2,6PGA were measured as copy number of HA cDNA by using real-time PCR. Each value (S.E.) is the average from three independent experiments. The general property for receptor binding specificities (preferential binding of avian IAV to Neu5Acα2,3Gal and of human IAV to Neu5Acα2,6Gal) was detected.