Fig. 3.
DHPG-induced phosphoinositide hydrolysis in Chinese hamster ovary (CHO) cells transiently transfected with mGluR5a wild-type (mGluR5a-WT) or mGluR5a-S890G DNA in the presence or absence of a calcineurin construct that is constitutively active (CaNa). There is a significant increase in response of cells transfected with mGluR5a-WT and CaNa and mGluR5a-S890G alone. As predicted, there is no further potentiation of responses in cells transfected with mGluR5a-S890G and CaNa. Data are represented as fold increase of no drug control measured 45 min after addition of 500 μM DHPG and are means ± S.E.M. of 5 experiments done in triplicate. An asterisk (*) indicates significant difference from response of cells transfected with mGluR5a-WT alone, p < 0.05 or less.