Skip to main content
. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Nat Neurosci. 2007 Apr 15;10(5):615–622. doi: 10.1038/nn1876

Figure 1.

Figure 1

Our two culture systems show well defined spinal motor neurons. (a,b) Immunostaining of primary neuronal cultures showing MAP2+HB9+ (a) and SMI32+HB9+ (b) large multipolar PMNs derived from nontransgenic mouse embryos. All were plated on NTgAML. (c,d) Double immunostaining of primary neuronal cultures showing MAP2+eGFP+ (c) and ChAT+eGFP+ (d) PMNs derived from a transgenic Hlxb9-GFP1Tmj embryo. (e–h) ESMNs expressing eGFP under the HB9 promoter cultured for 7 d on spinal cord astrocyte monolayers. Confirming their motor neuron phenotype, eGFP+HB9+ ESMNs are immunopositive for MAP2 (e,f), ChAT (g) and Islet (Isl) 1/2 (h). Scale bars, 50 μm (a–d,f–h) and 100 μm (e).