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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Regul Pept. 2013 Aug 17;186:92–103. doi: 10.1016/j.regpep.2013.08.001

Figure 10. Neuropeptide stimulation of keratinocyte inflammatory mediator expression requires ERK MAPK activation.

Figure 10

The ERK1/2 MAPK inhibitor PD98059 was used to demonstrate that SP and CGRP induced cytokine and NGF expression in keratinocytes required ERK1/2 activation. PD98059 concentration-dependently inhibited SP (10−8M, black bars) stimulation of TNF (A), IL-1 (B), IL-6 (C), and NGF (D) gene expression in keratinocytes at 3 h, measured by real time PCR. Similarly, PD98059 concentration-dependently inhibited CGRP (10−8M, black bars) stimulation of TNF (E), IL-6 (G), and NGF (H) gene expression in keratinocytes at 3 h, measured by real time PCR. There was no PD98059 inhibition of CGRP stimulation of IL-1 (F) expression, suggesting that other protein kinases or signaling pathways may mediate CGRP stimulation of IL-1 expression in keratinocytes. Values are means ±SE. * P<0.05, ** P<0.01, and *** P<0.001 vs vehicle treated control cells. # P<0.05, ## P<0.01, and ### P<0.001 vs SP or CGRP treated cells. These experiments were repeated 3-4 times.