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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Regul Pept. 2013 Aug 17;186:92–103. doi: 10.1016/j.regpep.2013.08.001

Figure 11. SP stimulation of keratinocyte inflammatory mediator expression requires ERK MAPK activation.

Figure 11

The JNK MAPK inhibitor SP600125 was used to demonstrate that SP induced cytokine and NGF expression in keratinocytes required JNK activation. SP600125 completely blocked SP (10−8M, black bars) stimulation of TNF (A), IL-1 (B), IL-6 (C), and NGF (D) gene expression in keratinocytes at 3 h, measured by real time PCR. CGRP treatment did not induce JNK activation (Fig. 7), thus CGRP was not tested against a SP600125. Values are means ± SE. * P<0.05, ** P<0.01, and *** P<0.001 vs vehicle treated control cells. # P<0.05, ## P<0.01, and ### P<0.001 vs SP treated cells. These experiments were repeated 3-4 times.