CGRP concentration-dependently up-regulated TNF-α, IL-1, IL-6, and NGF gene expression in keratinocytes at 3 h, as measured by real time PCR (A-D) and similarly increased cytokine and NGF protein secretion into the media over 24 hours after SP treatment, by EIA (E-H). Values are means ± SE. * P<0.05, ** P<0.01, and *** P<0.001 vs vehicle treated control cells. These experiments were repeated 3-4 times.