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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Regul Pept. 2013 Aug 17;186:92–103. doi: 10.1016/j.regpep.2013.08.001

Figure 6. Inflammatory mediator expression in keratinocytes required neuropeptide receptor activation.

Figure 6

SP (10−8M) induced up-regulation of cytokine and NGF gene expression in keratinocytes was concentration-dependently inhibited by the SP NK1 receptor antagonist LY303870, as measured by real time PCR at 3 h after SP treatment (A-D). Similarly, CGRP (10−8M) evoked up-regulation of cytokine gene expression in keratinocytes was concentration-dependently inhibited by the CGRP receptor antagonist CGRP8-37, as measured by real time PCR at 3 h after CGRP treatment (E-G). CGRP8-37 treatment had no significant effect on CGRP stimulated NGF gene expression in keratinocytes (H). Values are means ± SE. * P<0.05, ** P<0.01, and *** P<0.001 vs vehicle treated control cells. #P<0.05, ## P<0.01 and ### P<0.001 vs SP or CGRP treated cells. These experiments were repeated 3-4 times.