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. Author manuscript; available in PMC: 2014 Sep 10.
Published in final edited form as: Regul Pept. 2013 Aug 17;186:92–103. doi: 10.1016/j.regpep.2013.08.001

Figure 9. Neuropeptide stimulation of keratinocyte inflammatory mediator expression does not require p38 MAPK activation.

Figure 9

The p38 MAPK inhibitor SB203580 was used to demonstrate that SP and CGRP induced cytokine and NGF expression in keratinocytes does not require p38 activation. SB203580 had no effect on SP (10−8M, black bars) stimulation of TNF (A), IL-1 (B), IL-6 (C), and NGF (D) gene expression in keratinocytes at 3 h, measured by real time PCR. Similarly, SB203580 had no effect on CGRP (10−8M, black bars) stimulation of TNF (E), IL-1 (F), and NGF (H) gene expression in keratinocytes at 3 h, measured by real time PCR. SB203580 did inhibit CGRP stimulation of IL-6 (G) expression, suggesting that the p38 kinase activation may contribute to CGRP stimulation of IL-6 expression in keratinocytes. Values are means ± SE. * P<0.05, ** P<0.01, and *** P<0.001vs vehicle treated control cells. # P<0.05, ## P<0.01, and ### P<0.001 vs SP or CGRP treated cells. These experiments were repeated 3-4 times.