Fig. 5.

EM reconstruction of full-length talin. (A) Full-length talin1 adopts a compact shape with two-fold symmetry and volume consistent with two talin monomers. (B) Projections of the final, symmetrized reconstruction (M) correspond well to the corresponding non-symmetrized class average (C). (C) Representative raw particles, several of which show indications for 2-fold symmetry. (D) The distribution of particles in Euler angle space shows no preferred orientation. The bar in A represent 2.5 nm, the width of the boxes in B and C is 25 nm.