Table 2.
NMR screen for intra-molecular interactions in talin1.
| Unlabeled/15N labeled | F0–F3 | R1–R3 | R4–R8 | R9–R12 | R13–DD |
|---|---|---|---|---|---|
| F0–F1 | nb | nb | nb | nb | nb |
| F2–F3 | b | B | nb | B | nb |
| F3–R1 | nb | nb | B | B | nb |
| R2–R3 | nb | nb | nb | nb | nb |
| R3–R4 | nb | nb | nb | B | nb |
| R5–R6 | nb | nb | nb | nb | nb |
| R7–R8 | nb | nb | b | nb | nb |
| R9–R10 | B | nb | b | nb | nb |
| R11–R12 | nb | nb | b | b | b |
The 15N-labeled talin polypeptides (left hand column) were incubated with the unlabeled talin fragments shown in the top row. The domain composition of the polypeptides is shown. nb = no binding, B = binding, b = broadening of the spectra suggestive of a possible interaction, but not conclusive.