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. Author manuscript; available in PMC: 2013 Oct 19.
Published in final edited form as: Methods. 2008 Oct 21;46(3):213–223. doi: 10.1016/j.ymeth.2008.09.024

Table 2.

Instrumentation.

Technique Excitatin light source Excitation wave-
length selection		 Emission wavelength selection Detection
Fluorometry (PTI Delta RAM) Xe continuous 300–600 nm Monochromator Monochromator Photomultiplier
Fluorescence imaging (custom designed) Xe continuous Monochromator (PTI) Excitation filters (Lambda DG, Sutter or filter wheel, ASI) Emission filters (filter wheel) CCD camera (PXL, Photometrics or Pluto, Pixelvision)
Confocal imaging (BioRad Radiance 2002) Laser: 442 nm HeCd, 488 nm KrAr, 568 nm KrAr and 635 nm red diode na Emission filters (filter wheel) Photomultiplier